Ultra-multiplex CARS spectroscopic imaging with 1-millisecond pixel dwell time

Multispectral imaging microscope with millisecond time resolution.

A new multispectral imaging microscope with micrometer spatial resolution and millisecond temporal resolution has been developed. The imaging microscope is based on the use of an acousto-optic tunable filter (AOTF) for spectral tuning and a progressive scan camera capable of snapshot operation for recording. It can operate in two modes: images are recorded as a function of time or wavelength. W...

Bio-medical X-ray imaging with spectroscopic pixel detectors

The aim of this study is to review the clinical potential of spectroscopic X-ray detectors and to undertake a feasibility study using a novel detector in a clinical hospital setting. Detectors currently in development, such as Medipix-3, will have multiple energy thresholds allowing for routine use of spectroscopic bio-medical imaging. We have coined the term MARS (Medipix All Resolution System...

Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy

The fast and reliable characterization of pathological tissue is a debated topic in the application of vibrational spectroscopy in medicine. In the present work we apply multiplex coherent anti-Stokes Raman scattering (MCARS) to the investigation of fresh mouse brain tissue. The combination of imaginary part extraction followed by principal component analysis led to color contrast between grey ...

How spectroscopic x-ray imaging benefits from inter-pixel communication.

Spectroscopic x-ray imaging based on pixellated semiconductor detectors can be sensitive to charge sharing and K-fluorescence, depending on the sensor material used, its thickness and the pixel pitch employed. As a consequence, spectroscopic resolution is partially lost. In this paper, we study a new detector ASIC, the Medipix3RX, that offers a novel feature called charge summing, which is esta...

Spectroscopic Quantum Imaging Using Pixel-level Adcs in Semiconductor-based Hybrid Pixel Detectors